Allergome-wide peptide microarrays enable epitope deconvolution in allergen-specific immunotherapy

BackgroundThe interaction of allergens and allergen-specific IgE initiates the allergic cascade after crosslinking receptors on effector cells. Antibodies other isotypes may modulate such a reaction. Receptor requires binding antibodies to multiple epitopes allergen. Limited information is available complexity epitope structure most allergens.ObjectivesWe sought allow description IgE, IgG4, IgG recognition at global, allergome-wide level during immunotherapy (AIT).MethodsWe generated an microarray comprising 731 in form more than 172,000 overlapping 16-mer peptides. Allergen by was examined serum samples collected from subjects undergoing AIT against pollen allergy.ResultsExtensive induction linear peptide-specific Phl p 1– Bet v 1–specific humoral immunity demonstrated 3-year-long grass birch allergy, respectively. Epitope profiles differed between but were largely established already 1 year AIT, suggesting that dominant antibody clones remained as important contributors following their initial establishment early phase AIT. Complex, subject-specific patterns allergen isoform group cross-reactivities repertoires observed, indicate different levels protection sources.ConclusionsThe study highlights nature recognized We envisage deconvolution will be aspect future efforts describe analyze outcomes personalized manner. The allergens. (AIT). allergy. Extensive sources.